TABLE 2.

Results of PCR amplification of DNA from wildlife fecal samples followed by Southern analysis^a

Host	Siteb	Sample	SSU rDNA		DHFR		POLY(T)		B-TUB
			PCRc	Sequenced	PCRc	Sequenced	PCRc	Sequenced	PCRc	Sequenced
Calfe		GCH1P	+	2	+	2	+	2	+	2
Deer	S	506	+		−		+		+
Deer	S	524	+	3	−		−		+
Deer	S	540	∼		−		−		∼
Deer	B	553	+		−		−		−
Deer	S	563	+	3	−		−		+
Deer	S	569	+	3	−		−		−
Deer	S	570	+	3	−		−		−
Deer	S	571	+		−		−		−
Deer	C	576	+		−		−		−
Deer	S	578	∼		−		−		∼
Deer	S	586	+	4	−		−		∼
Deer	C	598	+	2	−		−		−
Skunk	C	4229	+		−		+		+
Raccoon	C	4250	+		−		−		−
Mouse	C	4227	+	4	+	2	+		+
Mouse	C	4252	∼		+	2	∼		−
Chipmunk	C	4234	+		+	2f	−		+
Chipmunk	C	4237	+	2	+	2	+	2	+
Chipmunk	C	4244	∼		+		−		−
Chipmunk	C	4247	+	4	+	2	+		−
Muskrat	C	589	+	4	−		∼		+
Muskrat	C	590	+	4	−		−		+
Muskrat	C	591	+	4	+	2	∼		+
Muskrat	C	592	+	4	−		−		+
Muskrat	C	593	+	4	−		−		+
Muskrat	C	603	+	4	+	2	+	—g	+	—g

^aA total of 10 of 91 deer samples, 1 of 2 skunk samples, 1 of 5 raccoon samples, 1 of 2 mouse samples, 3 of 5 chipmunk samples, and 6 of 6 muskrat samples were positive with the SSU primer set. Two deer samples, one mouse sample, and one chipmunk sample were provisionally positive with the SSU primer set. Samples that were negative for the SSU PCR products are not shown. 

^bS, Stanwood; B, Black Rock Forest; C, Calder Center. 

^cThe results of Southern analysis of PCR products from amplifications with primer pairs are indicated as follows: +, positive (signal corresponding to product within 5% of the expected size) ∼, provisional (signal very weak or corresponding to a product between 5 and 10% larger or smaller than expected); −, negative (no signal or signal corresponding to a product either more than 10% larger or more than 10% smaller than expected). 

^dThe genotypes of the cloned and sequenced PCR products were as follows: for SSU rDNA, C. parvum genotype 2 (16, 46) or C. parvum genotype 3 or 4 (this study) (Table 3; Fig. 2); for DHFR, C. parvum genotype 2 (39); for POLY(T) C. parvum genotype 2 (5); and for B-TUB, C. parvum genotype 2 (30). 

^eC. parvum genotype 2 reference positive control. 

^fAllelic variant. 

^gAlleles that are not attributed to a Cryptosporidium species or genotype.