TABLE 3.
Alignment of the hypervariable regions of the Cryptosporidium SSU rDNA PCR products from wildlife samples and reference sequencesa
| Source (sample) | Genotypeb | Sequence at positions 632 to 667c | Sequence at positions 678 to 698c |
|---|---|---|---|
| 598 | C. parvum genotype 2d | ATATAAAATATTTTGAT--GAATATT--TATATAATATTA | TATT-------ACTA--TATATTT----TAGTAT |
| 4237 | C. parvum genotype 2e | ATATAAAATATTTT-----GAATATT--TATATAACATTA | TATT-------ACTA--TATTTTT----TAGTAT |
| C. parvum ferret | ATATAAAATATTTTGAT--TAATATT--TATATAATATTA | TATT-------ACTA--AATTTTTGTT-TGGTAT | |
| C. parvum mouse | ATATAAAATATTTTAAT--TAATATT--TATATAATATTA | TATT-------ACTAT-AATTATTTTT-TAGTAT | |
| C. parvum genotype 1 | ATATAAAATATTTTGAT--GAATATT--TATATAATATTA | TATT-------ACTA--TTTTTTTTTT-TAGTAT | |
| C. meleagridis | ATATATAATATTT-GAT--TAATATT--TATATAATATTA | TATT-------ACTA--AATTTAT----TAGTAT | |
| C. wrairi | ATATATAATATTTTGA---AAATATT--TATATAATATTA | TATT-------ACTA--TATATTTT---TAGTAT | |
| 586 | Genotype 4 | ATATATAATATTTTA-T---GATATT--TATATAATATTA | TATT-------ACTAT---TATTATT---AGTAT |
| 591 | Genotype 4 | ATATATAATATTTTA-C---GATATT--TATATAATATTA | TATT-------ACTAT---TAT---T---AGTAT |
| 524 | Genotype 3 | ATATATAATATTTTATT---AATATT--TATATAGTATTA | TATT-------ACTATA---TTTTAT---AGTAT |
| 563 | Genotype 3 | ATATATAATATTTTATT---AATATT--TATATAGTATTA | TATT-------ACTAT----TTTAT----AGTAT |
| C. parvum pig | ATATATAATATTTT--T---AATATT--TATATAATATTA | TATT-------ACTATAATTTTTATT---AGTAT | |
| C. felis | ATATATAATATTTTTTTTTAAATATTATTATGTAAGATTA | TATTTTTTAAGACTGAATTTTTAGTTTTGATAAT | |
| C. parvum dog | ATATATAATATTTAA-----CATATT--TATATAATATTA | TATT-------ACTAT--TTAT-------AGTAT | |
| C. parvum kangaroo | ATATATTATACTTTTT----AAGGTGTTTATATAATATTA | TATT-------ACTATATTTTTTT-----AGTAT | |
| Cryptosporidium sp. | ATATATAATATTACG------GTATT--TATATAATATTA | TATT-------ACTTTATTTTTAG-----AGTAT | |
| ∗∗∗∗∗ ∗∗∗ ∗ ∗ ∗∗∗ ∗∗ ∗∗∗∗ | ∗∗∗∗ ∗∗∗ ∗∗ |
The sequences from wildlife fecal samples 586, 590, 4227, 4247, 603, 592, 589, and 593 are identical in the hypervariable region, as are the sequences from samples 524, 569, and 570. The accession numbers for the PCR products from wildlife fecal samples are listed in Materials and Methods, as are the accession numbers for reference sequences.
The genotypes of reference sequences are listed by number or name. Genotypes 3 and 4 are new genotypes (this study).
Nucleotide positions in the C. parvum SSU rRNA gene sequence (accession number L16996). Asterisks indicate nucleotide identity.
Type A SSU rDNA sequence (16).
Type B SSU rDNA sequence (16).