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. 2022 Jul 9;22:749. doi: 10.1186/s12885-022-09828-2

Fig. 4.

Fig. 4

Mido(L)-ATRA promotes the interactions between RAF and Lyn/Fgr/Hck and inhibits the activities of Lyn/Fgr/Hck. HL-60, HL-60Res and U937 cells were treated as indicated for 2, 1 and 1 h, respectively. (A) RAF was immunoprecipitated, followed by Western blot analysis of Lyn, Fgr and Hck. (B) Phospho-Src Y416 was analyzed by Western blotting in whole-cell lysates of the three cell lines. GAPDH was used as the internal control. (C) Fgr and Lyn in HL-60 and HL-60Res cells and Lyn, Fgr and Hck in U937 cells were immunoprecipitated, followed by Western blot analysis of phospho-Src Y416. The same membrane incubated with the antibody against phospho-Src Y416 was stripped, and detection of Lyn, Fgr and Hck was then performed. The full length blots are presented in Supplementary Fig. 3. The values shown below each lane indicate relative units, with the values in DMSO-treated cells defined as 1.0

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