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Effect of laccase on the size distribution of compounds with absorption at 280 nm (Abs280) during fermentation with S. cerevisiae transformants L+Ss (▴) and L−Ss (●). (A) GPC of fermentation medium after cultivation with 1.25 mM coniferyl aldehyde. Elution of the standards, coniferyl aldehyde (○) and coniferyl alcohol (Δ), is also shown. (B) GPC of a dilute acid hydrolysate of spruce at the end of fermentation. The inset shows a close-up for fractions 1 to 4.
