Table 1.
Intrinsic properties of layer V neurons
| Non-FS Sham | Non-FS TBI | p value | FS sham | FS TBI | p Value | PYR sham | PYR TBI | p Value | |
|---|---|---|---|---|---|---|---|---|---|
| Action potential properties | |||||||||
| Half width (m sec) | 0.56 (0.03) | 0.65 (0.02) | 0.0053 (unpaired t test) | 0.29 (0.01) | 0.27 (0.01) | 0.2574 (unpaired t test) | 0.86 (0.07) | 0.78 (0.04) | 0.2938 (unpaired t test) |
| Amplitude (mV) | 52.07 (2.90) | 55.00 (2.68) | 0.4808 (unpaired t test) | 53.75 (1.80) | 54.75 (2.01) | 0.7432 (unpaired t test) | 74.12 (2.26) | 75.79 (1.32) | 0.5154 (unpaired t test) |
| Threshold (mV) | −36.09 (1.18) | −36.18 (1.02) | 0.9529 (unpaired t test) | −38.61 (1.22) | −41.37 (1.52) | 0.1614 (unpaired t test) | −39.56 (1.38) | −37.53 (1.08) | 0.2491 (unpaired t test) |
| Rising slope (mV/s) | *191.8 (165.4–212.7) | *177.3 (162.4–236.5) | 0.7366 (Mann–Whitney) | 302.5 (17.21) | 320.5 (12.87) | 0.4253 (unpaired t test) | 301.8 (19.99) | 353.8 (13.73) | 0.0368 (unpaired t test) |
| Falling slope (mV/s) | −116.6 (9.38) | −94.68 (4.12) | 0.0530 (Welch's t test) | −252.2 (19.13) | −286.2 (16.77) | 0.2010 (unpaired t test) | −99.33 (11.03) | −92.38 (6.77) | 0.5855 (unpaired t test) |
| Spike AHP (mV) | 15.11 (1.41) | 13.42 (0.64) | 0.2323 (unpaired t test) | 22.61 (0.62) | 21.85 (1.01) | 0.5087 (unpaired t test) | 11.59 (0.69) | 15.59 (0.89) | 0.0015 (unpaired t test) |
| Adaptation index | 2.35 (0.16) | 3.25 (0.33) | 0.0226 (Welch's t test) | 1.40 (0.04) | 1.292 (0.04) | 0.0994 (unpaired t test) | *2.59 (2.51 - 3.94) | *2.43 (2.12 - 3.48) | 0.2777 (Mann–Whitney) |
| Rheobase (mV) | 69.76 (20.73) | 58.75 (16.76) | 0.6849 (unpaired t test) | 194.20 (31.67) | 194 (28.01) | 0.9971 (unpaired t test) | 86.31 (14.48) | 86.1 (12.32) | 0.9915 (unpaired t test) |
| Passive properties | |||||||||
| Resting potential (mV) | −61.3 (2.92) | −59.19 (1.77) | 0.5166 (unpaired t test) | −65.12 (1.40) | −66.71 (1.85) | 0.4899 (unpaired t test) | −64.53 (1.85) | −66.06 (1.48) | 0.5200 (unpaired t test) |
| Membrane resistance (MΩ) | 271.3 (37.06) | 279.2 (26.77) | 0.8618 (unpaired t test) | 140.6 (16.22) | 124.8 (13.34) | 0.4702 (unpaired t test) | 133.3 (16.37) | 130.8 (10.60) | 0.8961 (unpaired t test) |
| Tau (m sec) | 15.67 (2.18) | 19.08 (2.70) | 0.3826 (unpaired t test) | *7.55 (6.10 - 9.53) | *6.9 (5.79 - 8.54) | 0.4041 (Mann–Whitney) | 17.7 (1.77) | 20.97 (1.63) | 0.1836 (unpaired t test) |
Values are mean (SEM) or
*median (95% CI). Statistical test is indicated with associated p value. PYR= pyramidal neuron. Italics further denote p values. Bold values indicate significant p values.