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Experimental design for behavioral tests
| Condition | Drug (dose) | Vehicle | Route of injection | Test | Figure |
|---|---|---|---|---|---|
| Naive | MIF (50 ng) | Saline | Hindpaw | CPA | 9 A |
| Naive | MIF (50 ng) | Saline | Hindpaw | MC | 9B, C |
| SCI | Iso-1 (30 µg) | Saline/DMSO | Intrathecal | CPP | 9 D |
| Naive | Iso-1 (30 µg) | Saline/DMSO | Intrathecal | CPP | 9 E |
CPA, conditioned place avoidance; CPP, conditioned place preference; DMSO, dimethyl sulfoxide; MC, mechanical conflict ; MIF, macrophage migration inhibitory factor ; SCI, spinal cord injury.
