Table 3.
MIF has little effect on electrophysiological measures of excitability in RA neurons isolated from Naive, Sham, and SCI ratsa
| MIF (ng/ml) | 0 | 0.1 | 0.5 | 1 | 10 | Test | |
|---|---|---|---|---|---|---|---|
| Naive | RMP (mV) | –67.1 (–80.9, –55.3) (11) | –66.1 (–73.2, –59.8) (10) | –65.1 (–66.3, –62) (3) | –70.1 (–75.2, –52.6) (9) | –61 (–70, –55) (13) | BF |
| AP voltage threshold (mV) | –26 (–32.6, –12.2) (11) | –29.5 (–34.4, –19.4) (10) | –30.1 (–32.4, –21.8) (3) | –24.6 (–31.5, –7) (9) | –25.3 (–34, –4.7) (13) | KW | |
| Rheobase (pA) | 240 (40, 460) (11) | 175 (100, 280) (10) | 165 (100, 210) (3) | 180 (135, 360) (9) | 160 (40, 400) (13) | BF | |
| MIF (ng/ml) | 0 | 0.1 | 0.5 | — | — | ||
| Sham | RMP (mV) | –65.6 (–71.2, –59) (12) | –64.2 (–73.5, –57.2) (15) | –61.1 (–62.5, –60) (4) | — | — | KW |
| AP voltage threshold (mV) | –25.8 (–31.6, –14.8) (12) | –26.7 (–34.2, –16.5) (15) | –27.5 (–31.7, –25.1) (4) | — | — | KW | |
| Rheobase (pA) | 207.5 (65, 250) (12) | 215 (55, 480) (15) | 172.5 (140, 260) (4) | — | — | KW | |
| MIF (ng/ml) | 0 | 0.01 | 0.1 | 1 | — | ||
| SCI | RMP (mV) | –65.4 (–72.2, –60.3) (4) | –68.7 (–73.3, –64.1) (2) | –57.9 (–69.1, –46.4) (10) | –70.5 (–78.1, –52.8) (13) | — | BF |
| AP voltage threshold (mV) | –33.9 (–34.7, –19.2) (4) | –31.4 (–31.4, –31.4) (2) | –24.7 (–36.5, –11.3) (10) | –29 (–37, –23.6) (13) | — | BF | |
| Rheobase (pA) | 150 (65, 185) (4) | 145 (130, 160) (2) | 237.5 (100, 490) (10) | 200 (100, 420) (13) | — | KW |
aValues are reported as medians (minimum, maximum) (number of cells sampled). Statistical analysis was performed by comparing each dose of MIF against vehicle (0 ng/ml). Statistical tests: BF, Brown–Forsythe and Welch ANOVA test followed by Dunnett's T3 multiple comparison test; KW, Kruskal–Wallis test followed by Dunn's multiple comparison test.