Fig. 5.

MKLP2 loss causes aneuploidy and exacerbates CIN. (A) Graph depicting fraction of cells with chromosome number deviating from the mode (three chromosomes/cell) for four chromosomes (2, 7, 10 and 15). *P<0.05, **P<0.01, n>200 cells/condition from two independent experiments (Chi squared test). (B) Images of chromosome enumeration probes (red) from A. (C) Gene effect scores of CRC cell lines with CIN (MSS, n=25) and without CIN (MSI, n=16) when MKLP2 was depleted via RNAi. *P<0.05 (unpaired two-tailed t-test). (D) Gene effect scores of CRC cell lines with CIN (MSS, n=27) and without CIN (MSI, n=10) when MKLP2 was depleted via CRISPR. *P<0.05 (unpaired two-tailed t-test). Mean is highlighted in C,D.