Fig. 4.

Overexpression of miRNA-370 or -494 restores the EC barrier. (A,B) HUVECs transfected as described in Fig. 2A–C and seeded at 24 h after transfection at a density of 100,000 cells per well in 8W10E ECIS plates. Impedance was measured continuously. By altering the frequency, the overall barrier (A) and cell–cell contacts (B) can be distinguished over time. Continuous lines indicate the mean, dotted lines indicate s.e.m. (C,D) The area under the curve of experiments as in A and B was quantified between 24 h and 48 h, and groups were analyzed using a paired one-way ANOVA with Dunnett test. Six individual experiments were performed. (E) Gene expression in control and MEG8 GapmeR 1-treated HUVECs was obtained from previously published data (GSE186616; Kremer et al., 2022). miRNA-370 and miR-494 targets were predicted using online available tools (miRWalk, miRanda, RNA22 and TargetScan). The fold change of miRNA targets was compared to expression of all genes comparing MEG8 knockdown and control conditions. Groups were compared using a Kolmogorov–Smirnov test. Data are presented as mean±s.e.m. *P<0.05.