Fig. 3.

Salt alters the degree of methylation in loosely bound pectins. (A) Seven-day-old Arabidopsis Col-0 seedlings were treated for 1, 3 and 6 h with water (mock, ‘m’) or 100 mM NaCl (‘N’). From the AIRs, 5 μg sugars were extracted and treated with double-distilled water (native) or 0.1 M Na₂CO₃, pH 11, to obtain de-methylated HG (deM-HG). Dot blots was performed using 2F4 (n=3). (B) Five micrograms of sugars extracted from 6-day-old seedlings after 24 h treatment with water (m) or 100 mM NaCl (‘N’) were used to perform dot blots as in A. Results using the 2F4, LM20 and LM19 antibodies are shown (n=3). (C,D) Six-day-old seedlings of the Col-0, fer-4 and herk1 the1-4 (C) or Col-0 and mpk6-3 (D) lines were treated as in B. Sugars were extracted and 5 μg spotted on nitrocellulose membranes after 1 h incubation with double-distilled water (native) or Na₂CO₃, pH 11 (deM-HG). Dot blots were performed using the 2F4 antibody (n=3).