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. 2022 Jun 17;149(12):dev200330. doi: 10.1242/dev.200330

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© 2022. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 2. — Spermatogenesis of Prm1-deficient mice. (A) Mean testis weight of Prm1^+/+, Prm1^+/− and Prm1^−/− males (n=8-10). (B) Mean diameter of seminiferous tubules of Prm1^+/+, Prm1^+/− and Prm1^−/− mice (n=4); 25 tubules per mouse were evaluated. (C) Quantification of elongating spermatids per seminiferous tubule cross-section in Prm1^+/+, Prm1^+/− and Prm1^−/− males (n=3). Five tubules per mouse were evaluated. (D) Hematoxylin and Eosin staining of testis of Prm1^+/+, Prm1^+/− and Prm1^−/− males. Tubules at stages VII to VIII of the epithelial cycle with spermatozoa lining up at the edge of tubule lumen are marked by asterisks. (E) PAS staining of testis of Prm1^+/+, Prm1^+/− and Prm1^−/− males. Acrosomal structures are indicated by vermillion arrowheads. Data are mean±s.d. and were analyzed using a two-tailed, unpaired Student's t-test. Scale bars: 50 µm.