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. 2022 Jun 17;149(12):dev200330. doi: 10.1242/dev.200330

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© 2022. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 3. — Analysis of chromatin condensation and ROS-induced DNA damage in epididymal Prm1-deficient sperm. (A) Representative transmission electron micrographs of Prm1^+/+, Prm1^+/− and Prm1^−/− epididymal sperm. (B) Quantification of DNA condensation of epididymal sperm from Prm1^+/+, Prm1^+/− and Prm1^−/− males (n=3); 100 sperm per male were analyzed. (C) Transmission electron micrograph of Prm1^−/− epididymal sperm. (D) Agarose gel loaded with genomic DNA isolated from epididymal sperm of Prm1^+/−, Prm1^−/−, Prm2^+/−, Prm2^−/− and WT males separated by electrophoresis. Additional lanes loaded with ladders (L) were cut from the image. (E) Percentage of 8-OHdG-positive sperm on tissue sections of caput and cauda epididymis of Prm1^+/+, Prm1^+/− and Prm1^−/− mice (n=3). (F) Representative IF staining against 8-OHdG in testis, caput epididymis and cauda epididymis tissue sections from Prm1^+/+, Prm1^+/− and Prm1^−/− males. Data are mean±s.d. and were analyzed using a two-tailed, unpaired Student's t-test (*P<0.05; **P<0.005). Scale bars: 2 µm in A,C; 50 µm in F.