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. 2022 Jun 17;149(12):dev200330. doi: 10.1242/dev.200330

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© 2022. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 4. — Secondary effects on Prm1-deficient epididymal sperm. (A) EN staining: quantification of EN-negative sperm (%) from Prm1^+/+, Prm1^+/− and Prm1^−/− males (n=5). A minimum of 200 sperm per male were analyzed. (B) HOS test: quantification of HOS-positive sperm (%) from Prm1^+/+, Prm1^+/− and Prm1^−/− males (n=3). A minimum of 200 sperm per male were analyzed. (C) Nuclear head morphology analysis for Prm1^+/+, Prm1^+/− and Prm1^−/− sperm. Consensus shapes of sperm heads are depicted. Four males per genotype and a minimum of 100 sperm per animal were analyzed. (D) Quantification of motile and immotile sperm (%) from Prm1^+/+, Prm1^+/− and Prm1^−/− males (n=3). Data are mean±s.d. and were analyzed using a two-tailed, unpaired Student's t-test (*P<0.05; **P<0.005; ***P<0.001). ns, not significant.