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. 2022 Jun 17;149(12):dev200330. doi: 10.1242/dev.200330

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© 2022. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 5. — Differentially expressed genes in the testis and altered protein abundances in sperm in protamine-deficient males. (A) Number of differentially expressed genes subdivided into higher and lower expressed genes in testis of Prm1^+/− and Prm1^−/− males compared with WT males. (B) Venn diagram illustrating changes in abundances of proteins from sperm basic protein extractions of Prm1^−/−, Prm1^+/− and Prm2^−/− males compared with WT. Proteins that were more abundant are in bold. Non-bold proteins showed lower abundance compared with WT. (C) IHC stainings against histone H3 (red) and PRM2 (green) of Prm1^+/+, Prm1^+/− and Prm1^−/− caput epididymal tissue sections. DAPI (in gray) was used as the counterstain. The H3 stainings are additionally shown as single gray channel pictures. Scale bars: 50 µm.