Fig. 4. NEMEP deletion leads to early defects in mouse embryo chimeras.

a Scheme of blastocyst injection, transfer to pseudopregnant mouse, and collection of embryos at E7.5 (Created with BioRender.com). b Brightfield and green fluorescence (GFP) images of embryo chimeras including WT and Nemep^−/− ESCs recovered at E7.5. Scale bars, 100 µm. Confocal microscopy images of serially sectioned embryo chimeras, with WT ESCs and Nemep^−/− mESCs dissected at E7.5 depicting GFP (green), FOXA2 expression (red) (c) or T expression (red) (d), and nuclear (Hoechst33342, blue) localization. Scale bars, transverse section, 100 µm; high-magnification view, 20 µm. b–d: Data are representative of three independent experiments with similar results. Source data are provided as a Source Data file.