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Purification of flavin reductase from recombinant E. coli
| Purification step | Protein (mg) | Total activity (U) | Sp act (U/mg) | Purification (fold) | Yield (%) |
|---|---|---|---|---|---|
| Cell extract | 791 | 40,300 | 50.9 | 1 | 100 |
| Ammonium sulfate fractionation | 389 | 29,400 | 75.6 | 1.5 | 73.0 |
| Q-Sepharose | 132 | 19,600 | 148 | 2.9 | 48.6 |
| Butyl-Toyopearl | 73.3 | 9,500 | 130 | 2.6 | 23.6 |
