TABLE 2.
Plasmids used
| Plasmid | Description | Reference or source |
|---|---|---|
| pCR-SCRIPT Am+ | Direct cloning vector | Commercially available from Stratagene (9) |
| pERFORM-Z | pERFORM-C with cam resistance gene replaced by zeocin resistance gene | 2 |
| pLR1 | pCR-SCRIPT with marRAB 1.3 kb in MCSa | This study |
| pLR2 | As pLR1 with cam cloned into XcmI site of marA | This study |
| pLR3 | As pLR2 with gfp cloned into AgeI site of marA | This study |
| pLR4 | pERFORM-Z suicide plasmid with marA::gfp::cam cloned into MCS | This study |
a
MCS, multiple cloning site.