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. Author manuscript; available in PMC: 2023 May 19.
Published in final edited form as: Mol Cell. 2022 Apr 6;82(10):1894–1908.e5. doi: 10.1016/j.molcel.2022.03.018

Figure 1. Gene expression in the HOXD/miR-10b locus is repressed in the human brain and activated in glioma cells and tumors.

Figure 1.

A. Pol II ChIP-seq in HOXD locus, based on GBM track from ENCODE database (GSM808768). Gencode V19 was used for gene annotation.

B. Relative miR-10b expression in the brain and LGG. Illumina HiSeq data for the normal brain (n=5) and LGG (n=500) were retrieved from TCGA and visualized as boxplots. Unit: log2 (FPKM-UQ+1).

C. Relative miR-10b expression in the brain and GBM. Boxplot visualization of Illumina Human v2 MicroRNA expression BeadChip dataset (GEO number: GSE25632) for the normal brain (n=5) and GBM (n=103).

D. Expression of HOXD genes in human brain cortex, cerebellum, LGG, and GBM. TCGA TARGET GTEx dataset for cortex (n=300), cerebellum (n=300), LGG (n=500), and GBM (n=170) was visualized as boxplots. Unit: RSEM norm_count+1.

E. Relative miR-10b expression in normal neural cells, GSCs, and glioma lines was monitored by qRT-PCR. Each group included 3 biological replicates (mean+SD).

F. Relative expression of HOXD genes in normal neural and glioma cells was monitored by qRT-PCR (mean+SD, n=3).