. Author manuscript; available in PMC: 2022 Jul 10.

Published in final edited form as: Nat Methods. 2021 Dec;18(12):1427–1440. doi: 10.1038/s41592-021-01327-9

Table II:

Example utilization of the three axes matrix of the 4DN-BINA-OME Microscopy Metadata Specifications

Nr.	Description	Sample	Resolution	Image structure	Analysis	1st Axis Tier	2nd Axis OME extension	3rd Axis Requirement level
1	Investigation of PTC299 effect on SARS-CoV2 replication in Vero cells using widefield commercial microscope	Fixed cells	Low	Dual-channel; multi-point	Simple segmentation and intensity quantification	1	*C + B*	*Required only*
2	High-resolution OligoPaint investigation of chromatin structure using commercial widefield microscope	Fixed cells	High	Multi-channel; 3D Z-stack	Spot detection and subpixel localization	2	*C + B + CP*	*Required only*
3	High-resolution, real-time tracking of export of HIV-1 genomic RNA from infected T-cells using custom made microscope	Live cells	Super	Multi-channel; 3D Z-stack; time-series	Spot detection, subpixel localization and tracking	3	*ALL*	*ALL*
4	Widefield microscope manufacturers	Any	Any	Any	Any	3	*C + B + CP*	*ALL*

C, OME Core; B, 4DN-BINA-OME Basic Extension; AC, 4DN-BINA-OME Advanced & Confocal Extension; CP, 4DN-BINA-OME Calibration & Performance Extension