Figure 3. Effect of DATS treatment on actin cytoskeleton-associated genes.

(A) Expression of CASK, EEF1A1, GBP2, ITSN1, and UTRN in MCF-10A and SK-BR-3 cells after 16-hour treatment with DMSO (control) or 20 µM DATS. Results are shown as mean ± SD (n = 3) and statistical analysis was done by unpaired Student’s t-test. (B) qRT-PCR analysis of the genes shown in panel (A) in the SK-BR-3 cell line treated for 16 hours with DMSO (control) or the indicated doses of DATS. Combined results from two independent experiments are shown as mean ± SD (n = 6). Statistical analysis was done by one-way ANOVA followed by Dunnett’s test. qRT-PCR, quantitative real-time reverse transcription-PCR; SD, standard deviation; DMSO, dimethyl sulfoxide, DATS, diallyl trisulfide. *P < 0.05,