. 2022 Apr 19;5(2):329–340. doi: 10.1089/crispr.2021.0076

Table 2.

Off-target Cleavage Sites That Were Identified by TEG-seq Analysis and Validated by Targeted Amplicon-seq Analysis

graphic file with name crispr.2021.0076_inline2.jpg

MM bases are shown in bold, and the lower-case letters denote DNA bulges (i.e., DNA sequences with insertion compared to the guide RNA). Numbers in the gold background indicate the presence of indels detected by targeted Amplicon-seq analysis at the loci where the off-target cleavage were identified by TEG-seq analysis in the HEK293T-EGFR cells. Subject: egfp; OT1: chr7:139180275; OT2: chr6:52851930; OT3: chr15:96942561; OT4: chr1:51258089, OT5: chr12:123049729; OT6: chr19:46276033; OT7: chr3:55683731; OT8: chr14:104138952; OT9: chr1:44903194; OT10: chr8:139494509; OT11: chr17:61901954; OT12: chr8:108176664; OT13: chr2:197060859, OT14: chr10:104203223; OT15: chr12:108650920; OT16: chr1:49054155.

ND, not detected. Color images are available online.