Fig. 5.

CD8⁺ T cell accumulation into tumor 7 days after the initial light exposure. The specimens were assessed by multiplex IHC staining with anti-CD8 (magenta), anti-CD4 (green), anti-Foxp3 (yellow), and DAPI (blue). Photos show representative results of each treatment group. CD8⁺ cell (gray-filled arrowhead), CD4⁺Foxp3⁻ cell (white-filled arrowhead), and CD4⁺Foxp3⁺ cell (white open arrowhead) were counted. White dotted lines indicate tumor edges. Scale bar = 100 µm. Dot plots show the intratumoral CD8⁺ cell density in each experimental group (n = 5; one-way ANOVA followed by Tukey’s test; **, p < 0.01)