Skip to main content
. Author manuscript; available in PMC: 2023 Jul 1.
Published in final edited form as: J Allergy Clin Immunol. 2022 Jan 24;150(1):114–130. doi: 10.1016/j.jaci.2021.12.794

Fig 1. Efficient CHS requires NK1R, SP and HK1.

Fig 1.

A) Skin CHS to DNCB by ear thickness increase in WT and NK1RKO B6 mice. B) Microscopic images of the ears of mice in (A) showing epidermal-dermal thickness (lines), hyperkeratosis, parakeratosis, acanthosis and leukocyte inflammatory infiltrate. C) Skin CHS to DNCB, in WT, Tac1KO, Tac4KO and Tac1/Tac4KO B6 mice. D) Representative microscopic images of mouse ears in (C). E) CHS to DNCB in Tac1/Tac4KO B6 mice injected i.d. with SP or HK1, or SP and HK1 during sensitization. F) Microscopic images of the ears of mice in (E) showing epidermal-dermal thickness (lines) and leukocyte infiltrate. In (A), (C) and (E), representative experiment of 3. (A) Six mice per group, (B) and (C) 5 mice per group. Means ± 1 SD analyzed by 1-way ANOVA followed by Student-Newman-Keuls test. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. NS: not significant. Statistic comparisons among values obtained 48 h following ear skin elicitation are depicted. In (B), (D) and (F), images are from samples obtained 48 h after elicitation. H&E, X200, scale bars, 20μm.