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. 2022 Jun 27;13:887602. doi: 10.3389/fimmu.2022.887602

Figure 1.

Figure 1

Relative miR-33-5p expression level in MG-infected cells and chicken embryonic lung tissue. (A) The relative level of miR-33-5p in MG-infected DF-1 cells and CP-II cells. Cells cultivated in 6-well culture dishes were treated with MG. After 24h treatment, total RNA of infected cells were extracted using TRNzol Universal. The level of miR-33-5p-infected cells was detected by qPCR; (B) The relative level of miR-33-5p in MG-infected chicken embryo lungs. Total RNA were extracted from infected chicken embryo lungs on days 6-9 post-infection (equivalent to days 15-18 of eggs hatching) using TRNzol Universal. Then, the level of miR-33-5p in MG infected embryo chicken lungs was determined through qPCR. Above data was corrected via 5s-RNA as the internal quantitative control gene. All measurements shown were the mean ± SD from three independent experiments, each with three replicates. (*p < 0.05, **p < 0.01).