|
Bacterial Strains
|
| Turbo competent cells |
New England Biolabs |
Cat#C2984H |
| DH10Bac competent cells |
Thermo Fisher Scientific |
Cat#10361012 |
|
Chemicals, Peptides, and Recombinant Proteins
|
| Protease inhibitor cocktail |
MedChemExpress |
Cat#HY-K0010 |
| Lauryl maltose neopentyl glycol (LMNG) |
Anatrace |
Cat#NG310 |
| n-Dodecyl-β-D-Maltopyranoside (DDM) |
Anatrace |
Cat#D310S |
| Glyco-Diosgenin (GDN) |
Anatrace |
Cat#GDN101 |
| Cholesteryl hemisuccinate tris salt |
Anatrace |
Cat#CH210 |
| ESF 921 Medium |
Expression Systems |
Cat#96–001–01 |
| Freestyle 293 expression medium |
Gibco |
Cat#12338018 |
| DMEM/F-12 medium |
Gibco |
Cat#11330–032 |
| Penicillin-streptomycin solution, 100x |
Corning |
Cat#30–002-CI |
| Fetal bovine serum |
Gibco |
Cat#16140089 |
| Lipofectamine 2000 reagent |
Invitrogen |
Cat#11668019 |
| TransIT-Insect transfection reagent |
Mirus |
Cat#MIR6100 |
| 1,1-Methanediyl Bismethanethiosulfonate (MTS-1-MTS) |
Santa Cruz Biotech |
Cat#SC-208706 |
| 1,4-Butanediyl Bismethanethiosulfonate (MTS-4-MTS) |
Santa Cruz Biotech |
Cat#SC-208789 |
|
Deposited Data
|
| KV4.2/KChIP2 channel complex, open state |
This study |
PDB: 7UK5; EMDB: EMD-26575 |
| KV4.2/KChIP2 channel complex, inactivated state, class 1 |
This study |
PDB: 7UKC; EMDB: EMD-26576 |
| KV4.2/KChIP2 channel complex, inactivated state, class 2 |
This study |
PDB: 7UKD; EMDB: EMD-26577 |
| KV4.2/KChIP2 channel complex, intermediate state |
This study |
PDB: 7UKE; EMDB: EMD-26578 |
| KV4.2/KChIP2 channel complex, putative resting state |
This study |
PDB: 7UKF; EMDB: EMD-26579 |
| KV4.2/KChIP2/DPP6 channel complex, open state, transmembrane region |
This study |
PDB: 7UKG; EMDB: EMD-26580 |
| KV4.2/KChIP2/DPP6 channel complex, open state, intracellular region |
This study |
PDB: 7UKH; EMDB: EMD-26581 |
|
Experimental Models: Cell Lines
|
| HEK293 |
ATCC |
Cat#CRL-1573 |
| Sf9 |
ATCC |
Cat#CRL-1711 |
| HEK293S GnTI–
|
ATCC |
Cat#CRL-3022 |
|
Recombinant DNA
|
| pEG-BacMam Human Kv4.2EM
|
This study |
N/A |
| pEG-BacMam Human KChIP2 |
This study |
N/A |
| pEG-BacMam Human DPP6 |
This study |
N/A |
|
Software and Algorithms
|
| Serial EM |
Mastronarde, 2005
|
http://bio3d.colorado.edu/SerialEM
|
| CTFFIND4 |
Rohou and Grigorieff, 2015 |
http://grigoriefflab.janelia.org/ctffind4
|
| RELION |
Scheres, 2012 |
http://www2.mrclmb.cam.ac.uk/relion
|
| cryoSPARC |
Punjani et al., 2017
|
https://cryosparc.com/
|
| Coot |
Emsley et al., 2010
|
http://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot
|
| Phenix |
Adams et al., 2010 |
https://www.phenix-online.org
|
| MolProbity |
Williams et al., 2018 |
http://molprobity.biochem.duke.edu
|
| Chimera |
Pettersen et al., 2004
|
https://www.cgl.ucsf.edu/chimera
|
| PyMOL |
Schrodinger |
http://www.pymol.org
|
| pCLAMP |
Molecular Devices |
https://www.moleculardevices.com/
|
| Anaconda |
Anaconda |
https://www.anaconda.com/
|
|
Other
|
| CNBR-activated Sepharose beads |
Cytiva |
Cat#17043001 |
| Superose 6 Increase 10/300 GL SEC column |
Cytiva |
Cat#29091596 |
| R1.2/1.3 400 mesh UltrAuFoil gold grids |
Quantifoil |
Cat#Q350AR13A |
