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. Author manuscript; available in PMC: 2023 Jul 7.
Published in final edited form as: Mol Cell. 2022 May 24;82(13):2443–2457.e7. doi: 10.1016/j.molcel.2022.04.034

Figure 5. Expression of ARAF influences the biological consequences of ligand activated RAS signaling. See also Figure S5.

Figure 5.

(A) PC12 cells were transfected with indicated siRNAs for 48 hours and then treated with 50 ng/ml recombinant NGF. Images were taken at the indicated time points (left). Percentage of differentiated cells was quantified (right). Scale bar, 20 μM. Bars, mean±SD biological triplicate of experiments.

(B) PC12 cells transfected with indicated siRNAs (left) or expressing indicated constructs (right) were seeded in 96 well plates at a density of 2,000 cells/well and supplemented with or without 100 ng/ml EGF. Cell growth was measured. Bars, mean±SD biological triplicate of experiments. **** p<0.0001, two-tailed Student’s t test.

(C) PC12 cells expressing doxycycline-inducible A-, B- or C-RAF were treated with 100 ng/ml doxycycline for 24 hours. Cells were then treated with 100 ng/ml EGF. Scale bar, 20 μM. Bars, mean±SD biological triplicate of experiments.