FIGURE 6.

The SNM could promote the recruitment and survival of healthy volunteer-derived peripheral-blood lymphocytes in the subcutaneous tumors formed by MHCC97-H cells in mice. MHCC97-H cells were obtained by culture. Then, cells were inoculated subcutaneously into SCID mice to enable formation of tumor tissue. Simultaneously, peripheral-blood lymphocytes from healthy volunteers were infused back into SCID mice (∼1 × 10⁷ cells per mouse). Then, mice received sorafenib (1 mg/kg, p.o.) or a combination of sorafenib (1 mg/kg) + SNM (0.2 g/kg). Then, tumor tissue was collected, ground, and flow cytometry-sorted to determine the level in each 50 mg of tissue. The number of peripheral-blood lymphocytes recruited (CD45⁺ fraction) and the number of surviving peripheral-blood lymphocytes (CD45 combined with trypan-blue staining) (A) or survival of cells (B) are shown as histograms. (A) *p < 0.05 compared with control (recruitment of peripheral-blood lymphocytes); (B) #p < 0.05 compared with control (survival of peripheral-blood lymphocytes). *p < 0.05.