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. 2022 Jun 27;13:907042. doi: 10.3389/fpls.2022.907042

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Copyright © 2022 Escandón, Bigatton, Guerrero-Sánchez, Hernández-Lao, Rey, Jorrín-Novo and Castillejo.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Protease activity in 9% polyacrylamide gel slabs containing gelatin at different germination stages. Cotyledon samples (A). Embryo axis samples (B): Gels containing no inhibitor (1); an inhibitor of serine protease activity (PMSF; 2); and one of metalloprotease activity (EDTA; 3). NV: Nongerminated acorns after 4 weeks of germination. T1: mature acorns prior to germination. T2, T3, T4: Germinated acorns when root tips were visibly emerging from cotyledons (T2), their size reached 6.5 mm (T3) and it exceeded 20 mm (T4). Ten bands were cut from protease activity gels for shotgun analysis (red boxes).