Figure 1.

In vitro ApoB mRNA target reduction in primary mouse hepatocytes (PMHs) after 72 h of gapmer ASOs exposure

Reduction of target ApoB mRNA was quantified by qRT-PCR. All experiments are carried out in triplicates. (A) Design, antisense activity, Tm (melting temperature), and Rt (retention time) are shown. Positions of phosphorodithioate (PS₂) (yellow ^o) are shown while the rest of ASO is stereorandom PS modified. ASOs marked with asterisk (∗) were tested in a different experiment following the same screening condition. ^aMelting temperature to complementary RNA (Tm) is shown; complementary RNA for Tm measurement is as follows: UGAAUACCAAUGC. ^bRP-HPLC retention time (Rt) is shown. ^cUpper case designates LNA nucleotide and lower case designates DNA nucleotide, with symbols designating stereorandom PS (•), PS₂ (yellow ^o), and phosphate (red •). ^dStandard deviation (±) is shown; nd, not determined. (B) Dose-response curves for reducing ApoB mRNA in PMHs are shown.