TABLE 1.

Distribution and lipid classes of fatty acids produced from linoleic acid by washed cells of L. acidophilus under microaerobic conditions^a

Fatty acid	Fatty acid concn (mg/ml of reaction mixture) after reaction	Distribution of fatty acids in indicated lipid class (mol%) in:
		Supernatant			Cells			Total
		FAc	AGd	PLe	FA	AG	PL
Linoleic acid	0.24	1.9	0.3	—f	2.7	0.3	—	5.2
CLA	3.76	3.5	0.6	—	74.5	3.9	—	82.5
HY	0.04	—	—	—	0.6	0.3	—	0.9
Other fatty acidsb	0.52	1.4	0.3	—	8.6	1.1	trg	11.4
Total	4.56	6.8	1.2	—	86.4	5.6	tr	100

^aL. acidophilus was cultivated in MRS medium with linoleic acid (0.1%) for 3 days. The reaction was carried out with 5 mg of linoleic acid per ml as the substrate for 3 days under the conditions described in Materials and Methods. The results are averages of three separate determinations that were reproducible within ±10%. 

^bOther lipids (moles percent) were myristic acid (0.1), palmitic acid (1.5), palmitoleic acid (0.1), oleic acid (7.3), vaccenic acid (1.7), and 2-hexy-1-cyclopropane-octanoic acid (0.7). 

^cFA, free fatty acids. 

^dAG, acylglycerols. 

^ePL, phospholipids. 

^f—, not detected. 

^gtr, trace (less than 0.05 mol%).