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. 2001 Mar;67(3):1253–1261. doi: 10.1128/AEM.67.3.1253-1261.2001

TABLE 1.

Bacterial strains and plasmids

Strain or plasmida Origin or relevant characteristics Source and/or reference
Bacterial strains
 L. acidophilus ATCC 4356 Type strain; human pharynx ATCCb
 L. acidophilus NCFM Human intestinal isolate 7
 L. casei ATCC 393 Type strain; cheese isolate ATCC
 L. delbrueckii subsp. bulgaricus 1489 Cheese isolate 55
 L. gasseri ADH Human intestinal isolate 30 (called strain MS02)
 L. gasseri ATCC 33323 Type strain; human isolate ATCC
 L. gasseri NCK 1338 Human endoscopy isolate 32
 L. gasseri NCK 1340 Human endoscopy isolate 32
 L. gasseri NCK 1341 Human fecal isolate 32
 L. gasseri NCK 1342 Human endoscopy isolate 32
 L. gasseri NCK 1343 Human fecal isolate 32
 L. gasseri NCK 1344 Human endoscopy isolate 32
 L. gasseri NCK 1345 Human endoscopy isolate 32
 L. gasseri NCK 1346 Human endoscopy isolate 32
 L. gasseri NCK 1347 Human endoscopy isolate 32
 L. gasseri NCK 1348 Human fecal isolate 32
 L. gasseri NCK 1349 Human fecal isolate 32
 L. helveticus CNRZ 32 Cheese isolate Steelec (8)
 L. johnsonii VPI 11088 ATCC 11506; origin unknown VPId
 L. plantarum 299v Human intestinal isolate 1
 L. reuteri DSM 20016 ATCC 53609, type strain; human fecal isolate ATCC
 L. rhamnosus GG ATCC 53103; human fecal isolate ATCC
 E. coli DH10B F−mcrA Δ(mrr-hsdRMS-mcrBC) φ80dlacZΔM15 ΔlacX74 endA1 recA1 deoR Gibco-BRLe
 E. coli DH5α F− φ80dlacZΔM15 Δ(lacZYA-argF) U169 endA1 recA1 hsdR17 (rK−mK+) deoR Gibco-BRL
 E. coli KW1 Δ(add-gus-man) hsdR hsdM+metB strA purB 54
 E. coli Tuner(DE3) F−ompT hsdSB (rB−mB−) gal dcm lacY1 (DE3) Novagenf
Plasmids
 pET28a(+) Kanr; E. coli expression vector Novagen
 pTRK665 pET28a(+) with cloned gusA gene This study
 pUC18 Apr; cloning vector Amershamg
 pTRK666 pUC18 with 4.5-kb L. gasseri ADH chromosomal insert; GusA+ This study
 pTRK667 pUC18 with 4.9-kb L. gasseri ADH chromosomal insert; GusA+ This study
 pGK12 ori (pWV01), Emr, Cmr; gram-positive shuttle vector 31
 pTRK563 Emr; Δcat-derivative of pGK12 with lacZ from pBluescript II KS(+) This study
 pTRK668 pTRK563 with 4.5-kb EcoRI-PstI insert from pTRK666; GusA+ This study
 pTRK664 pTRK563 with T7 terminator-P6 promoter-gusA gene; GusA+ This study
a

Intermediate plasmids not shown. 

b

American Type Culture Collection. 

c

James Steele, Dept. of Food Science, University of Wisconsin—Madison. 

d

Virginia Polytechnic Institute. 

e

Gibco-BRL, Grand Island, N.Y. 

f

Novagen, Inc., Madison, Wis. 

g

Amersham Pharmacia Biotech, Piscataway, N.J.