Fig. 1.

Circadian expression of the master clock molecules in the suprachiasmatic nucleus. (A) The region of interest to examine the immunoreactivities of respective proteins in the suprachiasmatic nucleus is shown as a white lining on the brain section stained using 4’,6-diamidino-2-phenylindole (DAPI) (left). The representative photos of immunohistochemistry using Bmal1 (upper) and Per2 (lower) antibodies are shown according to the zeitgeber time (ZT) in wild-type (WT) and P301S mice (middle). The image analysis of diurnal changes of Bmal1 and Per2 immunoreactivities shows a significant difference at ZT20 in Bmal1 in wild-type vs. P301S tau mice (right). Scale bars, 100 μm. (B) The quantitative reverse transcription (qRT)-PCR data reveal a discrepancy between wild-type and P301S mice in the ZT-dependent level changes of Bmal1 and Per2 transcripts. Data are mean±SEM (n=3). *p<0.05 and ^#p<0.1 on Mann-Whitney test.