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Purification of CHB3. S. lividans TK21(pWF17) was grown in the presence of glucose. The proteins from the culture filtrate (lane 1) and the highly purified CHB3 protein (lane 2) were analyzed by SDS-PAGE. The purified CHB3 protein was also tested for cross-reactions with anti-CHB1 antibodies (lane 3). Size markers are given (in kilodaltons).
