TABLE 1.

Binding specificity of CHB3^a

Substrate	% Binding specificity
	Unbound	Loosely bound	Bound
Chitin
Hen lobster tendon (α-chitin)	0	60	40
Crab shell (α-chitin)	0	97	3
Squid pen (β-chitin)	0	93	7
Colloidal chitin	77	23	0
Chitosan	0	100	0
Avicel	100	0	0
Glucan	100	0	0

^aCHB3 was incubated with each substrate at pH 6 at room temperature overnight. After centrifugation, the proteins in the supernatant were analyzed by SDS-PAGE and immunodetected (unbound). The proteins which bound to the substrates were then eluted by the buffer containing 1 M NaCl, separated by SDS-PAGE, and immunodetected (loosely bound). Proteins associated with the pellets were released by boiling (95°C, 10 min) and, after removal of the insoluble substrate, analyzed by SDS-PAGE and subsequent immunodetection (bound). After a corresponding photo was scanned, the quantity of cross-reacting protein was related to the total amount of CHB3.