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. Author manuscript; available in PMC: 2022 Jul 11.

Published in final edited form as: FASEB J. 2021 Oct;35(10):e21889. doi: 10.1096/fj.202100036R

Oxysterol cytoprotection depends partly on NR1H3 and NR1H2. Leakage of LDH and viability of epithelial (A) and stromal (B) cells transfected for 48 h with scramble siRNA or siRNA targeting NR1H3, NR1H2, or both NR1H3 and NR1H2; treated with vehicle or 25 ng/ml 27-hydroxycholesterol for 24 h; and, then challenged for 2 h with control medium () or pyolysin (, epithelium 200 HU, stroma 25 HU). Data are presented as mean (SEM) using cells from 4 independent animals; statistical significance is determined using two-way ANOVA and Tukey’s post hoc test

Inline graphic — Oxysterol cytoprotection depends partly on NR1H3 and NR1H2. Leakage of LDH and viability of epithelial (A) and stromal (B) cells transfected for 48 h with scramble siRNA or siRNA targeting NR1H3, NR1H2, or both NR1H3 and NR1H2; treated with vehicle or 25 ng/ml 27-hydroxycholesterol for 24 h; and, then challenged for 2 h with control medium () or pyolysin (, epithelium 200 HU, stroma 25 HU). Data are presented as mean (SEM) using cells from 4 independent animals; statistical significance is determined using two-way ANOVA and Tukey’s post hoc test