TABLE 1.
Oxysterol concentrations in biological samples
| Oxysterol | Uterus (ng/ml) | Growing follicle (ng/ml) | Dominant follicle (ng/ml) |
|---|---|---|---|
| 22R-hydroxycholesterol | 0.2 ± 0.2 | 2.3 ± 0.8 | 2.4 ± 1.5 |
| 24S-hydroxycholesterol | 0.2 ± 0.2 | 0.2 ± 0.1 | 0.5 ± 0.1 |
| 25-hydroxycholesterol | 3.9 ± 0.6 | 13.4 ± 10.5 | 26.8 ± 11.4 |
| 25-hydroxycholest-4-en-3-one | 0.0 ± 0.0 | 0.1 ± 0.1 | 2.1 ± 1.8 |
| 27-hydroxycholesterol | 19.9 ± 4.9 | 104.3 ± 69.7 | 244.6 ± 99.5 |
| 27-hydroxycholest-4-en-3-one | 0.0 ± 0.0 | 0.5 ± 0.2 | 6.8 ± 5.2 |
| 7β-hydroxycholesterol | 0.8 ± 0.8 | 0.4 ± 0.2 | 0.4 ± 0.2 |
| 7α-hydroxycholesterol | 8.3 ± 4.9 | 0.9 ± 0.6 | 1.1 ± 0.0 |
| 7α-hydroxycholest-4-en-3-one | 17.0 ± 8.4 | 0.2 ± 0.1 | 1.3 ± 1.1 |
| 6β-hydroxycholesterola | 7.1 ± 0.9 | 2.3 ± 0.6 | 2.2 ± 0.2 |
| 6β-hydroxycholest-4-en-3-onea | 0.8 ± 0.5 | 0.2 ± 0.1 | 0.9 ± 0.6 |
| 7-oxocholesterol | 13.2 ± 1.5 | 2.8 ± 1.0 | 6.8 ± 5.1 |
| 24,25-epoxycholesterol | 0.6 ± 0.6 | 7.5 ± 4.9 | 9.3 ± 7.3 |
| 24,25-epoxycholest-4-en-3-one | 0.0 ± 0.0 | 0.3 ± 0.2 | 6.6 ± 5.3 |
| 24,27-dihydroxycholesterol | 0.5 ± 0.5 | 5.8 ± 3.0 | 4.8 ± 3.0 |
| 24,27-dihydroxycholest-4-en-3-one | 0.0 ± 0.0 | 0.3 ± 0.2 | 2.6 ± 2.3 |
| 7α,25-dihydroxycholesterol | 1.3 ± 0.7 | 0.7 ± 0.4 | 2.0 ± 1.5 |
| 7α,25-dihydroxycholest-4-en-3-one | 1.5 ± 0.4 | 1.5 ± 0.5 | 16.7 ± 12.6 |
| 7α,27-dihydroxycholesterol | 0.0 ± 0.0 | 0.2 ± 0.2 | 0.0 ± 0.0 |
| 7α,27-dihydroxycholest-4-en-3-one | 2.3 ± 0.7 | 1.3 ± 0.2 | 13.8 ± 6.4 |
| 3β,7β-dihydroxycholest-5-en-26-oic acid | 0.0 ± 0.0 | 2.3 ± 0.2 | 2.0 ± 1.0 |
| 7β-hydroxy-3-oxocholest-4-en-26-oic acid | 0.0 ± 0.0 | 0.3 ± 0.17 | 0.5 ± 0.2 |
| 3β,7α-dihydroxycholest-5-en-26-oic acid | 4.9 ± 2.5 | 13.2 ± 6.9 | 6.5 ± 4.2 |
| 7α-hydroxy-3-oxocholest-4-en-26-oic acid | 81.3 ± 20.0 | 76.8 ± 21.0 | 258.9 ± 136.8 |
| 3β,22R-dihydroxycholest-5-en-26-oic acid | 0.0 ± 0.0 | 23.5 ± 14.7 | 23.9 ± 13.5 |
| 22R-hydroxy-3-oxocholest-4-en-26-oic acid | 0.0 ± 0.0 | 3.0 ± 1.6 | 11.1 ± 4.7 |
| 20R,22R,25-trihydroxycholesterol | 0.9 ± 0.9 | 10.7 ± 5.5 | 2.4 ± 1.8 |
| 20R,22R,25-trihydroxycholest-4-en-3-one | 1.1 ± 0.8 | 2.4 ± 0.7 | 39.5 ± 21.3 |
| 7α,24,25-trihydroxycholest-4-en-3-one | 4.8 ± 2.5 | 4.3 ± 1.3 | 20.5 ± 13.2 |
| 3β-hydroxycholest-5-enoic acid | 12.7 ± 6.8 | 283.3 ± 249.4 | 295.8 ± 118.4 |
| 3-oxocholest-4-enoic acid | 4.4 ± 3.1 | 20.8 ± 12.9 | 11.1 ± 4.7 |
| 3β,7α,24-trihydroxycholest-5-en-26-oic acid | 0.0 ± 0.0 | 0.57 ± 0.57 | 0.0 ± 0.0 |
| 7α,24-dihydroxy-3-oxocholest-4-en-26-oic acid | 3.6 ± 1.8 | 5.1 ± 0.2 | 16.4 ± 6.8 |
| 3β,7α,25-trihydroxycholest-5-en-26-oic acid | 0.0 ± 0.0 | 0.8 ± 0.6 | 1.5 ± 0.9 |
| 7α,25-dihydroxy-3-oxocholest-4-en-26-oic acid | 2.5 ± 1.4 | 3.1 ± 1.1 | 12.3 ± 6.3 |
Note: Concentrations of oxysterols in samples of uterine fluid or follicular fluid from growing (4 to 8 mm diameter) or dominant (>8 mm diameter) ovarian follicles (data are reported as mean ± SEM, n = 3 per sample).
a
Dehydration products of cholestane-3β,5α,6β-triol.