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. 2022 Jul 8;31:09636897221108705. doi: 10.1177/09636897221108705

Figure 3.

Figure 3.

Dopamine and Sul-121 concentration-dependently prevent DNA strand breaks in cooled and rewarmed SMAC. (A, B) Typical examples of alkaline and neutral comets at 40× magnification. Noncooled control SMAC (37°C), SMAC cooled for 24 h at 4°C (C), and cooled SMAC with an additional rewarming for 4 h at 37°C (C/R4) were examined in the presence of vehicle, dopamine (Dopa) and Sul-121. (C) Quantification of DNA strand breaks under alkaline (SSB+DSB) and neutral (DSB) conditions in noncooled control cells (37°C) and cells cooled for 24 h at 4°C (“Cooled”) and cooled cells following an additional rewarming for 4 h at 37°C (“Rewarmed”) in absence and presence of dopamine and Sul-121. Dose response curves are mean and range (25th and 75th percentile). (D, E) Pulsed-field gel electrophoresis of C/R4 SMAC in the absence or presence of dopamine (Dopa; 30 μM) or Sul-121 (100 nM). a, b, c denote differences from other groups. C/R: cooling and rewarming; DSB: double-strand break; SSB: single-strand break.