Skip to main content
. Author manuscript; available in PMC: 2022 Jul 11.
Published in final edited form as: Neuron. 2021 Jan 20;109(5):823–838.e6. doi: 10.1016/j.neuron.2020.12.023

Figure 3. FC depressed CeA synapses on PBN neurons.

Figure 3.

(A) Schematic showing injection of AAV-ChR2(H134R)-eYFP into the CeA of WT mice.

(B and C) Representative coronal brain sections showing the injection site with ChR2(H134R)-eYFP expression in the CeA (B) and the CeA terminals expressing ChR2(H134R)-eYFP in PBN, but not in LC (C). The CeA also projects to the lateral habenula (LHb).

(D–G) Representative recordings of CeA IPSC (postsynaptic neuron held at 0 mV, black trace) in PBN (F), but not in LC (E). The IPSC was blocked by GABAzine (held at 0 mV, blue trace). A summary of the percentages of cells responding to CeA stimulation is shown in (G).

(H–J) Depression of CeA synapses (ChR2 in H and I and Chronos in J) on PBN after FC (H and J) and after FC-consolidation-reconsolidation (I). Example traces are shown in the upper panels.

(K and L) Representative traces (K) and summary of paired-pulse ratio (PPR) of CeA-Chronos IPSCs in PBN showing an increase of PPRs in FC mice (L).

Scale bars: 1 mm in (B); 200 μM in (C); 200 pA and 20 ms in (E), (F), and (H)–(J); 50 pA and 20 ms in (K). Statistics: **p ≤ 0.005, Mann-Whitney U test. BLA, basolateral amygdala; LPBN, lateral PBN; MPBN, medial PBN; scp, superior cerebellar peduncle; 4V, fourth ventricle. Also see Figure S4 and Table S1.