KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Tyrosine Hydroxylase (TH) mouse monoclonal antibody | Immunostar | 22941; RRID: AB_572268 |
| Tyrosine Hydroxylase (TH) sheep polyclonal antibody | Pel-Freez | P60101-150; RRID: AB_461070 |
| CGRP goat polyclonal antibody | Abcam | 36001; RRID: AB_725807 |
| GFP rabbit polyclonal antibody | ThermoFisher | A11122; RRID: AB_221569 |
| goat anti-mouse Alexa 594 | ThermoFisher | A11032; RRID: AB_2534091 |
| goat anti-sheep Alexa 488 | ThermoFisher | A11015; RRID: AB_2534082 |
| donkey anti-goat Alexa 350 | ThermoFisher | A21081; RRID: AB_2535738 |
| goat anti-rabbit Alexa 488 | ThermoFisher | A11034; RRID: AB_2576217 |
| Bacterial and virus strains | ||
| AAV2/9-hSyn-hChR2(H134R)-eYFP | UNC vector core | N/A |
| AAV9-Syn-Chronos-GFP | UNC vector core | N/A |
| AAV9-EF1a-DIO-hChR2(H134R)-eYFP | UNC vector core | N/A |
| AAV9-Syn-ChrimsonR-tdTomato | UNC vector core | N/A |
| AAV8-hSyn-DIO-hM3D(Gq)-mCherry | UNC vector core | N/A |
| AAV8-hSyn-DIO-hM4D(Gi)-mCherry | UNC vector core | N/A |
| AAV5-hSyn-Con/Fon-hChR2(H134R)-eYFP-WPRE | UNC vector core | N/A |
| AAV9-CMV-Cre-2A-tdTomato | Virovek (Hayward, CA) | N/A |
| AAV9-U6-Control-shRNA-CMV-tdTomato-WPRE-bGHpA | Virovek (Hayward, CA) | N/A |
| Chemicals, peptides, and recombinant proteins | ||
| GABAzine | Tocris | 1262 |
| DNQX | Tocris | 2312 |
| D-AP5 | Tocris | 0106 |
| MPEP | Tocris | 1212 |
| CPCCOEt | Tocris | 1028 |
| Win | Tocris | 1038 |
| AM251 | Tocris | 1117 |
| Rimonabant | Tocris | 0923 |
| Noradrenaline | Abcam | Ab120717 |
| Phenylephrine | Tocris | 2838 |
| Isoproterenol | Tocris | 1747 |
| HEAT | Tocris | 0535 |
| Biocytin | Sigma | B4216 |
| CNO | Hello Bio | HB6149 |
| Cy5-streptavidin | ThermoFisher | 434316 |
| Experimental models: organisms/strains | ||
| Mouse: C57BL/6J | The Jackson Laboratory | RRID: IMSR_JAX:000664 |
| Mouse: CRF-cre: B6;FVB-Tg(Crh-cre) 1Kres/J | The Jackson Laboratory | RRID: IMSR_JAX:011087 |
| Mouse: TH-cre: B6.Cg-7630403G23RikTg(Th-cre)1Tmd/J | The Jackson Laboratory | RRID: IMSR_JAX:008601 |
| Mouse: vGlut2-Cre: Slc17a6tm2(cre)Lowl/j | The Jackson Laboratory | RRID: IMSR_JAX:016963 |
| Mouse: TH-2A-Flp | Poulin et al., 2018 | N/A |
| Mouse: vGlut2-cre; TH-2A-Flp | Poulin et al., 2018 | N/A |
| Mouse: Ai65: B6;129S-Gt(ROSA) 26Sortm65.1(CAG-tdTomato)HZe/J | The Jackson Laboratory | RRID: IMSR_JAX:021875 |
| Mouse: vGlut2-cre; TH-2A-Flp; Ai65 | Dr. Rajeshwar Awatramani This paper | N/A |
| Mouse: CB1Rlox/lox | Marcus et al., 2020 | N/A |
| Oligonucleotides | ||
| crn1 | ThermoFisher | Mm01212171_s1 |
| Gapdh | ThermoFisher | Mm99999915_g1 |
| Software and algorithms | ||
| Clampex & Clampfit 10.7 | Molecular Devices Inc. | RRID: SCR_011323 |
| FreezeFrame 5 | Actimetrics | RRID: SCR_014429 |
| Igor Pro 6.32 | WaveMetrics | RRID: SCR_000325 |
| Prism 6 | GraphPad Software Inc. | RRID: SCR_002798 |
| IMARIS 8.2 | Bitplane | RRID: SCR_007370 |
| Other | ||
| Standard chow | Teklad | 7912 |
| Grain pellet | Bio-Serv | F0163 |
| Chocolate pellet | Bio-Serv | F05301 |