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. 2022 Jul 8;42(7):BSR20221181. doi: 10.1042/BSR20221181

Figure 2. Knockdown of EFR3 impairs insulin-stimulated 2DG transport.

Figure 2

(A) 3T3-L1 adipocytes were electroporated with siRNA designed to knockdown EFR3A or PI4K-IIIα, or scrambled control siRNA (Scr) at day 6 post-differentation as described. Cells were incubated in serum-free media for 2 h, with or without 100 nM insulin for the last 30 min then 2DG uptake measured. Because cell numbers varied considerably between experiments, the data are normalized to the rate obtained in the Scr-cells in the absence of insulin. Data shown as mean and SEM of data from at least three biological repeats, each with five technical replicates. Knockdown of either EFR3 or PI4K-IIIα impaired insulin-stimulated 2DG uptake (*P<0.001 for both). Differences in basal transport rate were not significant. (B) shows a representative immunoblot in which levels of EFR3 and PI4K-IIIα, together with GAPDH, are shown in lysates of cells treated with the indicated siRNA. Data from a representative experiment is shown.