Fig. 3.

Isolation of MPXV-IMBmuc1 on Vero E6 cells and serology results. VeroE6 cells were inoculated with sample material from a lanced pustule of Patient #1 following standard procedure [17]. A Typical plaque formation of MPXV IMBmuc 1 on Vero E6 2 days post-infection of the sample material. B Vero E6 mock infected. C Plaque morphology of vaccinia virus (VACV) Elstree (1), VACV WR (2), MPXV IMBmuc 1 (3; most similar to VACV Elstree), MPXV IMBdrc 2510 (4), on MA104 cells 3 dpi—size bar refers to (1–4). D Reactivity of VACV Elstree in immunofluorescence assay with sera from patient #1 at hospital admission (1; non-reactive) and 11 days later (2; reactive, titre 80), sera of MVA unvaccinated (3; non-reactive), and MVA vaccinated (4; reactive) controls