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(A) Growth of strain PAO1 in Basal-Salt medium (BSM) supplemented with 5 mM succinate and 40 mM mannitol. The arrows indicate the times of sample withdrawal during carbon catabolite repression (CCR; T1), at the beginning of the lag-phase (T2) and after relief of CCR (T3). (B) The steady state levels of the regulatory RNA CrcZ were monitored by Northern blot analysis at T1, T2, and T3, respectively. 5S RNA was used as a loading control.
