Figure 2.

Global fitting of amide CSPs of EIN against a series of HPr concentrations. The ¹⁵N HSQC spectra of 200 μM ²H/¹⁵N EIN were recorded in dilute buffer (A), in 10% (w/v) Ficoll-70 (B), and in 10% (w/v) BSA (C), respectively. The K_D values calculated using the CSPs of L19, K30, F65, G110, and G134 are 2.82 ± 3.14 μM in a dilute environment, 2.45 ± 2.52 μM in a Ficoll-70 environment, and 3.02 ± 3.12 μM in a BSA environment.