Figure 4.

Treatment of patient-derived CD8 + T cells with 9-ING-41 increases granzyme B, TRAIL, and IFN-gamma secretion while decreasing VEGF, TNF-alpha, and CCL5/RANTES concentrations in cell culture supernatant in comparison to control groups. Patient-derived CD8+ cytotoxic T cells were treated with 9-ING-41 for 48 hours (1 µM) or control (DMSO) and cytokine concentration was measured in cell culture supernatants. (a) Violin plots representing cytokine concentrations that increased post-treatment. (b) Violin plots representing cytokine concentrations that decreased post-treatment. Statistical significance was calculated with GraphPad Prism 9.3.1 using unpaired t tests and is denoted on each plot as follows: P > .05 = n.s., P ≤ .05 = *, P ≤ .01 = **, P ≤ .001 = ***, and P ≤ .0001 = **** (N = 3).