Figure 1.

m⁶A-signal dissipates first followed by clearance of the SG cores at later time point. Time course of SG disassembly in U2OS-G3BP1-GFP cells monitored by confocal microscopy. Cells were pre-exposed to 500 µM AS for 30 min and allowed to recover in permissive growth medium and. Zero min denotes the time point of medium exchange and withdrawal of AS. SGs were visualized by G3BP1–GFP (green), m⁶A-modified mRNAs with m⁶A antibodies (red), nuclei were counterstained with DAPI (blue). Insets on the left, zoomed in area depicted on the merged image designated with dashed-line squares and a dot (left corner) in the corresponding colour. Scale bar, 10 µm.