Table 1.
Gene | Forward primer | Reverse primer |
---|---|---|
18s | 5′-TGAAATATCCAGAACATCTTA-3′ | 5′-GCAAAATTTATTGTCCCATCAT-3′ |
CRP | 5′-GTGTTTCCCAAAGAGTCGGATA-3′ | 5′-CCACGGGTCGAGGACAGTT-3′ |
SAA1 | 5′-ATCAGCGATGCCAGAGAGAAT-3′ | 5′-GTGATTGGGGTCTTTGCCA-3′ |
SAA2 | 5′-AGCCAATTACATCGGCTCAG-3′ | 5′-ATTTATTGGCAGCCTGATCG-3′ |
SAA4 | 5’-GTCCAACGAGAAAGCTGAGG-3’ | 5’-AGTGACCCTGTGTCCCTGTC-3’ |
MCP1 | 5´-CCCCAGTCACCTGCTGTTAT-3´ | 5´-TGGAATCCTGAACCCACTTC-3´ |
NF-κB1 | 5´-CCTGGATGACTCTTGGGAAA-3´ | 5´-TCAGCCAGCTGTTTCATGGTC-3´ |
MMP9 | 5´-TTGACAGCGACAAGAAGTGG-3´ | 5´-GCCATTCACGTCGTCCTTAT-3´ |
MMP12 | 5´-ACACATTTCGCCTCTCTGCT-3´ | 5´-CCTTCAGCCAGAAGAACCTG-3´ |
IL-8 | 5´-CAGGAATTGAATGGGTTTGC-3´ | 5´-AAACCAAGGCACAGTGGAAC-3´ |
18s: 18s ribosome; CRP: C-reactive protein; SAA: Serum Amyloid A; MCP1: Monocyte chemoattractant protein 1; NF-κB1: Nuclear factor kappa-B1; MMP: Matrix Metalloproteinase; IL-8: Interleukin-8.