FIG. 3.

Phylogenetic dendrogram constructed with 16S rDNA sequences (about 750 bp) showing the relationship of RSa and RSb clones to members of the genus Clostridium (sensu Collins et al. [9]). The tree was constructed with the neighbor-joining method of the ARB program package and the Jukes-Cantor correction. To omit highly variable regions within the 16S rRNA, a filter with 50% invariance for the genus Clostridium was used. Scale bar, 10% estimated difference in nucleotide sequence position. As the outgroup, Bacillus subtilis was used.