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. 2022 Jun 30:eabl9943. doi: 10.1126/sciimmunol.abl9943

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Copyright © 2022 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works

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Fig. 3. — (A) Neutralization of Spike pseudoviruses that correspond to CDC VBMs and VOC in the presence of IMM20190/184/253 and (B) IMM20185/253 combination. Denoted points are Means of four replicates. Error bars denote SD. (C) Neutralization of D614G and Delta Spike pseudoviruses by the IMM-BCP-01 cocktail and S309 antibodies. Shown data are representative experiments of two independent repeats. (D) Synergy heatmaps (top) and scores (bottom) of the IMM-BCP-01 cocktail against five pseudoviruses and one BavPat1/2020 (D614G) live virus isolate calculated with SynergyFinder 2.0 online tool. HSA, the highest single agent model score, calculates the excess over the maximum single antibody response. Heatmaps (top) depict concentration-dependent HSA distribution, negative HSA in red and positive HSA in green. HSA score (bottom) below -10 indicates competition; between -10 to 10 shows additive effect; and above 10 demonstrates synergy among tested agents. (E) Neutralization of authentic VBM isolates of SARS-CoV-2 by the IMM-BCP-01 cocktail as measured in ViroSpot assay. Antibodies are mixed at equimolar ratio. Denoted points are Means of four replicates. Error bars denote SD. (F) Comparison of potency of the IMM-BCP-01 cocktail against the Alpha variant measured by three different methods, including a pseudovirus neutralization, intact virus spot reduction (ViroSpot) and intact virus plaque formation assays. (G) Neutralization of Omicron BA.1 and Omicron BA.2 (H) virus isolates by IMM20184, IMM20253 and IMM20184/253 combination as measured by plaque reduction assay. Denoted points are Means of four replicates. Error bars denote SD.