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. 2022 Jun 28;18(6):e1010272. doi: 10.1371/journal.pgen.1010272

Fig 7. Immunocytochemical staining of H2A.L.2 and PRM2 as well as TNP1 and unprocessed PRM2.

Fig 7

(A) (a-f) Immunocytochemical fluorescent staining of H2A.L.2 (red) and PRM2 (green) in WT, Prm2+/Δc and Prm2-/Δc step 15–16 spermatids from tubule preparations. Counterstained with DAPI (blue). (g-l) Immunocytochemical fluorescent staining of TNP1 (red) in WT, Prm2+/Δc and Prm2-/Δc step 15–16 spermatids from tubule preparations. Counterstained with DAPI (pseudo-colored grey or blue). (m-r) Immunocytochemical fluorescent staining of cP2 indicating the PRM2 precursor (red) in WT, Prm2+/Δc and Prm2-/Δc step 15–16 spermatids from tubule preparations. Counterstained with DAPI (pseudo-colored grey or blue). Scale bar = 20μm. (B) Immunoblots against GFP and TNP1 of GFP pull-down assay of HEK293 cells co-transfected with cP2-NLS-eGFP (pCP2-NLS-eGFP-N3) and untagged TNP1 (pTnp1-STOP-mCherry-N1) or eGFP (pEGFP-N3) and untagged TNP1. Immunoblot was repeated for cP2-NLS-eGFP and untagged TNP1 co-transfected samples (lower blot).