Figure 5.

CS-SeNPs inhibit Marc-145 cell apoptosis caused by r-PRRSV-EGFP. Marc-145 cells were infected with r-PRRSV-EGFP (MOI= 0.1) for 1 h and then incubated with or without 10 μM CS-SeNPs for 24 or 48 h. (A) ROS levels were detected by DHE fluorescence intensity. (B) Flow cytometry analysis of apoptosis rate of Marc-145 cells stained with Annexin V-PE at 48 hpi. (C) Quantitative analysis of apoptotic cells expressed as the mean ± SD of triplicate experiments. (D) Protein expression of p-JNK, JNK, caspase-3, cleaved-caspase-3, cleaved-PARP, and PRRSV-N in Marc-145 cells were evaluated by Western blot at 24 and 48 hpi. β-actin was used as the loading control. (E) Protein expression was quantified by Western blot and expressed as fold change relative to β-actin. All results are represented as the means ± SD of triplicate experiments. *P<0.05, **P <0.01 compared with mock, #P<0.05, ##P <0.01 compared with r-PRRSV-EGFP infected cells.

Abbreviations: CS-SeNPs, chitosan-coated selenium nanoparticles; DHE, dihydroethidium; hpi, hours post infection; MOI, multiplicity of infection; JNK, reactive oxygen species; PARP, poly adenosinediphosphate-ribose polymerase; N, nucleocapsid; ROS; reactive oxygen species; SD, standard deviation.